Journal of Biomaterials and Nanobiotechnology

Muriel Bonnet ¹, Christophe Massard ², Philippe Veisseire ¹, Camares Olivier ¹, Komla Oscar Awitor ²

Affiliations
1 Clermont Universite, Universite d'Auvergne, Laboratoire de Biologie, Aurillac Cedex, FR
2 Clermont Universite, Universite d'Auvergne, C-BIOSENSS, Clermont-Ferrand Cedex, FR

Abstract

The aim of this work was to evaluate the photokilling efficiency of synthesized titanium dioxide nanoparticles in suspension. Two strains of Escherichia coli, Lactobacillus casei rhamnosus and Staphylococcus aureus were used as probes to test the photokilling activities of the nanoparticles. The toxicity effects of TiO₂ nanoparticles on the environment were determined by a standard test using gram-negative bioluminescent bacteria Vibrio fischeri. The antimicrobial activity of these nanoparticles (NPs) was then investigated versus NPs concentration, UV irradiation time and micro- organism strains. We evaluated the LC50 values of the nanoparticles suspension by counting the Colony-Forming Units. Results highlighted the differences in bacteria sensitivity facing photokilling treatment induced by the irradiation of anatase TiO₂ nanoparticles suspension. At the concentration of 1 g·L⁻¹ TiO₂, tested bacteria were killed after 30 minutes of photo-treatment. Using different TiO₂ concentrations, the Staphylococcus aureus gram-positive/catalase-positive bacteria were more resistant than gram-negative/catalase-positive ones or gram-positive/catalase-negative bacteria. An effect of UV irradiation was evaluated by the quantification of hydrogen peroxide generated by the photolysis of water molecules in presence of the nanoparticles with or without the most resistant bacterium (S. aureus). After 30 minutes with UV irradiation in these two conditions, the concentration of hydrogen peroxide was 35 μM in presence of 1.2 g·L⁻¹ TiO₂ suspension. This result suggested that the resistance mechanism of S. aureus was not due to an extracelullar H₂O₂ enzymatic degradation.

Keywords

Photokilling Activity, Titanium Nanoparticles, E. coli, L. c. rhamnosus, S. aureus, Hydrogen Peroxide.

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